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Medientyp:
Buch
Titel:
Photoswitching proteins
:
methods and protocols
Enthält:
Photoinduced damage resulting from fluorescence imaging of live cells
/ Matthew K. Daddysman, Michael A. Tycon, and Christopher J. Fecko
Photoinduced damage resulting from fluorescence imaging of live cells
/ Matthew K. Daddysman, Michael A. Tycon, and Christopher J. Fecko
Modification of purified proteins with photochemical protection compounds for high-resolution photoactivation of protein function in vitro and in vivo
/ Sidney Cambridge
Optochemical activation of kinase function in live cells
/ Andrei V. Karginov, Klaus M. Hahn, and Alexander Deiters
Photoswitching of cell surface receptors using tethered ligands
/ Andreas Reiner and Ehud Y. Isacoff
Photocontrol of AMPA receptors with a photochromic ligand
/ Martin Sumser and Philipp Stawski
Photoconversion of CFP to study neuronal tissue with electron microscopy
/ Nina Wittenmayer
Light-inducible gene regulation with engineered zinc finger proteins
/ Lauren R. Polstein and Charles A. Gersbach
Manipulation of plasma membrane phosphoinositides using photoinduced protein-protein interactions
/ Olof Idevall-Hagren and Pietro DeCamilli
Roadmap to applying optogenetics in neuroscience
/ Consuelo Fois, Pierre-Hugues Prouvot, and Albrecht Stroh
Salvaging ruins : reverting blind retinas into functional visual sensors
/ Marion Mutter, Natalia Swietek, and Thomas A. Münch
Photoactivated adenylyl cyclases as optogenetic modulators of neuronal activity
/ Wagner Steuer Costa, Jana Liewald, and Alexander Gottschalk
Structural basis of photoswitching in fluorescent proteins
/ Chenxi Duan ... [et al.]
Using photoactivatable GFP to track axonal transport kinetics
/ Archan Ganguly and Subhojit Roy
In vivo cell tracking using PhOTO zebrafish
/ William P. Dempsey, Hanyu Qin, and Periklis Pantazis
In vivo optogenetics for light-induced oxidative stress in transgenic zebrafish expressing the killerred photosensitizer protein
/ Cathleen Teh and Vladimir Korzh
Photoactivatable fluorescent proteins for super-resolution microscopy
/ Yuji Ishitsuka, Karin Nienhaus, and G. Ulrich Nienhaus
pcSOFI as a smart label-based superresolution microscopy technique
/ Benjamien Moeyaert and Peter Dedecker.
Modification of purified proteins with photochemical protection compounds for high-resolution photoactivation of protein function in vitro and in vivo
/ Sidney Cambridge
Optochemical activation of kinase function in live cells
/ Andrei V. Karginov, Klaus M. Hahn, and Alexander Deiters
Photoswitching of cell surface receptors using tethered ligands
/ Andreas Reiner and Ehud Y. Isacoff
Photocontrol of AMPA receptors with a photochromic ligand
/ Martin Sumser and Philipp Stawski
Photoconversion of CFP to study neuronal tissue with electron microscopy
/ Nina Wittenmayer
Light-inducible gene regulation with engineered zinc finger proteins
/ Lauren R. Polstein and Charles A. Gersbach
Manipulation of plasma membrane phosphoinositides using photoinduced protein-protein interactions
/ Olof Idevall-Hagren and Pietro DeCamilli
Roadmap to applying optogenetics in neuroscience
/ Consuelo Fois, Pierre-Hugues Prouvot, and Albrecht Stroh
Salvaging ruins : reverting blind retinas into functional visual sensors
/ Marion Mutter, Natalia Swietek, and Thomas A. Münch
Photoactivated adenylyl cyclases as optogenetic modulators of neuronal activity
/ Wagner Steuer Costa, Jana Liewald, and Alexander Gottschalk
Structural basis of photoswitching in fluorescent proteins
/ Chenxi Duan ... [et al.]
Using photoactivatable GFP to track axonal transport kinetics
/ Archan Ganguly and Subhojit Roy
In vivo cell tracking using PhOTO zebrafish
/ William P. Dempsey, Hanyu Qin, and Periklis Pantazis
In vivo optogenetics for light-induced oxidative stress in transgenic zebrafish expressing the killerred photosensitizer protein
/ Cathleen Teh and Vladimir Korzh
Photoactivatable fluorescent proteins for super-resolution microscopy
/ Yuji Ishitsuka, Karin Nienhaus, and G. Ulrich Nienhaus
pcSOFI as a smart label-based superresolution microscopy technique
/ Benjamien Moeyaert and Peter Dedecker.
Anmerkungen:
Includes bibliographical references and index
Beschreibung:
"... focuses on proven applications of photoswitching proteins in cell and neurobiology. Optical approaches have been mostly embraced by the neurosciences to allow fast, high-resolution characterization and manipulation of single cells in complex neuronal tissue. These tools, including photoswitching of proteins, are now successfully used in almost all fields of biological research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Photoswitching Proteins: Methods and Protocols highlights many of the possibilities for research based on light-inducible, high-resolution manipulation of biological systems."--P. [4] of cover. --