Enthält:
Identification of protein secretion systems in bacterial genomes using MacSyFinder / Sophie S. Abby and Eduardo P.C. Rocha
Protein sorting prediction / Henrik Nielsen
Cell fractionation / Melissa Petiti, Laetitia Houot, and Denis Duché
Defining lipoprotein localisation by fluorescence microscopy / Maria Guillermina Casabona, Mylène Robert-Genthon, Didier Grunwald, and Ina Attrée
Identification of lipoproteins using globomycin and radioactive palmitate / Nienke Buddelmeijer
Defining membrane protein localization by isopycnic density gradients / Rhys A. Dunstan, Iain D. Hay, and Trevor Lithgow
Cell surface exposure / Anna Konovalova
Probing inner membrane protein topology by proteolysis / Maxence S. Vincent and Eric Cascales
Mapping of membrane protein topology by substituted cysteine accessibility method (SCAM) / Mikhail Bogdanov
Defining membrane protein topology using pho-lac reporter fusions / Gouzel Karimova and Daniel Ladant
In vivo and in vitro protein-peptidoglycan interactions / Gang Li and S. Peter Howard
Measure of peptidoglycan hydrolase activity / Yoann G. Santin and Eric Cascales
Protein-protein interaction : Bacterial Two-Hybrid / Gouzel Karimova, Emilie Gauliard, Marilyne Davi, Scot P. Ouellette, and Daniel Ladant
Protein-protein interactions : yeast two-hybrid system / Jer-Sheng Lin and Erh-Min Lai
Protein-protein interactions : cytology two-hybrid / Krishnamohan Atmakuri
Fusion reporter approaches to monitoring transmembrane helix interactions in bacterial membranes / Laureen Logger, Abdelrahim Zoued, and Eric Cascales
Protein-protein interactions : co-immunoprecipitation / Jer-Sheng Lin and Erh-Min Lai
Protein-protein interaction : tandem affinity purification in bacteria / Julie P.M. Viala and Emmanuelle Bouveret
Site-directed and time-resolved photocrosslinking in cells metabolically labeled with radioisotopes / Raffaele Ieva
Protein-protein interactions : pull-down assays / Arthur Louche, Suzana P. Salcedo, and Sarah Bigot
Protein-protein interactions : surface plasmon resonance / Badreddine Douzi
Assessing energy-dependent protein conformational changes in the TonB system / Ray A. Larsen
Defining assembly pathways by fluorescence microscopy / Abdelrahim Zoued and Andreas Diepold
Large complexes : cloning strategy, production, and purification / Eric Durand and Roland Lloubes
Shearing and enrichment of extracellular type IV pili / Alba Katiria Gonzalez Rivera and Katrina T. Forest
Blue native PAGE analysis of bacterial secretion complexes / Susann Zilkenat, Tobias Dietsche, Julia V. Monjarás Feria, Claudia E. Torres-Vargas, Mehari Tesfazgi Mebrhatu, and Samuel Wagner
In situ imaging of bacterial secretion systems by electron cryotomography / Gregor L. Weiss, João M. Medeiros, and Martin Pilhofer
Structural analysis of protein complexes by cryo electron microscopy / Tiago R.D. Costa, Athanasios Ignatiou, and Elena V. Orlova
Bacterial filamentous appendages investigated by solid-state NMR spectroscopy / Birgit Habenstein and Antoine Loquet
Energy requirements for protein secretion via the flagellar type III secretion system / Marc Erhardt
Identification of effectors : precipitation of supernatant material / Nicolas Flaugnatti and Laure Journet
Screening for secretion of the type VI secretion system protein Hcp by enzyme-linked immunosorbent assay and colony blot / Brent S. Weber, Pek Man Ly, and Mario F. Feldman
Effector translocation : cya reporter assay / Suma Chakravarthy, Bethany Huot, and Brian H. Kvitko
Monitoring effector translocation using the TEM-1 beta-lactamase reporter system / Julie Allombert, Anne Vianney, and Xavier Charpentier
Effector translocation assay : differential solubilization / Irina S. Franco, Sara V. Pais, Nuno Charro, and Luís Jaime Mota
Quantitative determination of anti-bacterial activity during bacterial co-culture / Juliana Alcoforado Diniz, Birte Hollmann, and Sarah J. Coulthurst.
Umfang:
xv, 528 Seiten; Illustrationen; 25.4 cm x 17.8 cm
Sprache:
Englisch
ISBN:
9781493970315;
1493970313
Verlags-, Produktions- oder Bestellnummern:
Sonstige Nummer: 978-1-4939-7031-5
Sonstige Nummer: 86413235