Enthält:
Detection and identification of uncapped RNA by ligation-mediated reverse transcription polymerase chain reaction
/ C. Kiong Ho
Preparation of splicing competent nuclear extract from mammalian cells and in vitro pre-mRNA splicing assay
/ Maliheh Movassat, Hossein Shenasa, and Klemens J. Hertel
Single-molecule analysis of pre-mRNA splicing with colocalization single-molecule spectroscopy (CoSMoS)
/ Joerg E. Braun and Victor Serebrov
In vitro modulation of endogenous alternative splicing using splice-switching antisense oligonucleotides
/ Jeong Eun Park and Luca Cartegni
Purification of mRNA processing complexes using an RNA affinity approach
/ Xiuye Wang and Yongsheng Shi
Polyadenylation site-based analysis of transcript expression by 3'READS+
/ Dinghai Zheng and Bin Tian
Comprehensive identification of mRNA polyadenylation sites by PAPERCLIP
/ Hun-Way Hwang and Robert B. Darnell
Microinjection and fluorescence in situ hybridization assay for studying mRNA export in mammalian cells
/ Ke Wang, Min Shi, and Hong Cheng
Methods for the detection of adenosine-to-inosine editing events in cellular RNA
/ Eimile Oakes, Pranathi Vadlamani, and Heather A. Hundley
Using RNA-Seq to discover genetic polymorphisms that produce hidden splice variants
/ Shayna Stein, Emad Bahrami-Samani, and Yi Xing
Inducible expression of eukaryotic circular RNAs from plasmids
/ Deirdre C. Tatomer, Dongming Liang, and Jeremy E. Wilusz
Methods for extraction of RNA, proteins, or protein complexes from subcellular compartments of eukaryotic cells
/ Kyu-Hyeon Yeom and Andrey Damianov
Isolation of newly transcribed RNA using the metabolic label 4-thiouridine
/ Angela Garibaldi, Francisco Carranza, and Klemens J. Hertel
Robust, cost-effective profiling of RNA binding protein targets with single-end enhanced crosslinking and immunoprecipitation (seCLIP)
/ Eric L. Van Nostrand, Thai B. Nguyen, Chelsea Gelboin-Burkhart, Ruth Wang, Steven M. Blue, Gabriel A. Pratt, Ashley L. Louie, and Gene W. Yeo
Purification of transcript-specific mRNP complexes formed in vivo from Saccharomyces cerevisiae
/ Jenna E. Smith and Kristian E. Baker
Cell-based high-throughput method for identifying modulators of alternative splicing
/ Sika Zheng
Genome-wide RNAi screens for RNA processing events in Drosophila melanogaster S2 cells
/ Natoya Peart and Eric J. Wagner
Assaying RNA structure inside living cells with SHAPE
/ Chao Feng, Dalen Chan, and Robert C. Spitale.